Edgewood Chemical Biological Center U.s. Army Soldier and Biological Chemical Comma.vd Ecbc-tr-058 Literature Review of Dna-based Subspecies Analysis of Bacillus Anthracis, Burkholderia Pseudomallei, Burkholderia Mallei, and Yersinia Pestis
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Design and Engineering of a Multi-target (multiplex) Dna Simulant to Evaluate Nulceic Acid Based Assays for Detection of Biological Threat Agents
We designed and engineered a non-infectious Biothreat simulant that included the nucleic acid signature of Bacillus anthracis, Yersinia pestis, Coxiellla Burneti, Brucella sp., Francicella tularensis, Entherohemorragic E. coli, O157:H7, Burkholderia mallei, Burkholderia pseudomallei and Variola virus (smallpox virus). A chimera of 2040 bp was engineered to produce PCR amplicons of different siz...
متن کاملConstruction and characterization of stable, constitutively expressed, chromosomal green and red fluorescent transcriptional fusions in the select agents, Bacillus anthracis, Yersinia pestis, Burkholderia mallei, and Burkholderia pseudomallei
Here, we constructed stable, chromosomal, constitutively expressed, green and red fluorescent protein (GFP and RFP) as reporters in the select agents, Bacillus anthracis, Yersinia pestis, Burkholderia mallei, and Burkholderia pseudomallei. Using bioinformatic approaches and other experimental analyses, we identified P0253 and P1 as potent promoters that drive the optimal expression of fluoresce...
متن کاملAntimicrobial Activity of CEM-101 a New Macrolide, Tested Against Diverse Collections of Bacterial Biowarfare/Bioterrorism (BW/BT) Agents
There continues to be a feared scenario of terrorist attacks with aerosolized microorganisms leading to mass infections. Given the added possibility of resistance to current treatments through genetic engineering or natural emergence, identifying effective antibiotics with novel mechanisms of action is critical to counter such an attack. In this study, we determined the minimum inhibitory conce...
متن کاملThe Potential of TaqMan Array Cards for Detection of Multiple Biological Agents by Real-Time PCR
The TaqMan Array Card architecture, normally used for gene expression studies, was evaluated for its potential to detect multiple bacterial agents by real-time PCR. Ten PCR assays targeting five biological agents (Bacillus anthracis, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis) were incorporated onto Array Cards. A comparison of PCR performance of...
متن کاملBurkholderia mallei and Burkholderia pseudomallei as bioterrorism agents: national aspects of emergency preparedness.
• Vol 9 • July 2007 B. mallei and B. pseudomallei as Bioterrorism Agents Burkholderia mallei and Burkholderia pseudomallei are the causative organisms of glanders and melioidosis, respectively. Although now rare in western countries, both organisms have recently gained much interest because of their unique potential as bio terrorism agents [1]. Despite being unique organisms, B. mallei and B. ...
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